Question: Promoters, operators, UASs, enhancers, and insulators are all examples of cis-regulatory elements…

Promoters, operators, UASs, enhancers, and insulators are all
examples of cis-regulatory elements.

a) True

b) False

If a mutation occurred that moved the operator of the lac operon
upstream of the promoter, then the lac operon would be
non-inducible.

a) True

b) False

Golden rice is an example of scientists using transgenic plants
to prevent vitamin A deficiency. The bacterial CRTI gene
(responsible for continuous production of beta-carotene, a vitamin
A precursor) was inserted into the rice endosperm.

a) True

b) False

A genetic strategy that starts with knowing the DNA sequence and
works toward identifying an associated phenotype is known as
forward genetics.

a) True

b) False

If recombination results in random integration of a DNA sequence
so that it produces a non-homologous region, the recombination is
legitimate.

a) True

b) False

Often, in the absence of experimental data, computerized
algorithms are used to predict gene structures from large
sequences. What is this approach known as bioinformatics.

a) True

b) False

If an entire genome is fragmented and then a large number of
pieces are sequenced, the approach is known as the shotgun
approach.

a) True

b) False

Genome size and gene number is highly correlated.

a) True

b) False

The purpose of creating cDNA from mRNA is to annotate a
genome.

a) True

b) False

The following questions deal with the lacZ gene
transformation experiment.

The Multiple Cloning Site (MCS) is located within the lacZ
gene.

a) True

b) False

All colonies observed growing on the X-gal/ampicillin plate are
the result of successful cloning (a piece of DNA was inserted into
the MCS).

a) True

b) False

If the cloning was unsuccessful, B-galactosidase will still be
produced which interacts with the X-gal on the plate to produce
blue colonies.

a) True

b) False