Question: Suppose you are engineering a mouse with a homozygous knockout of gene M as dicussed in chapter 1…
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Show transcribed image text Suppose you are engineering a mouse with a homozygous knockout of gene M as dicussed in chapter 10(gene replacement). You have completed many steps in the process and now, you are screening mouse pups for those that are heterozygous for the knockout – i.e., mice that have the wild type M allele on one chromosome, and the disrupted m allele on the other chromosome. To test a given mouse, you extract its genomic DNA, cut it with EcoRI, and perform a southern blot. A diagram of the wild type and knockout chromosomes is shown below. The position where the probe hybridizes is also shown. Your southern analysis includes mice of all possible genotypes – MM, Mm, and mm. Draw the banding pattern you would expect for each genotype on blot A below. Suppose the probe hybridizes only to the inserted DNA (the grey box on m chromosome). Draw the banding pattern you would expect for each genotype on blot B above.
Suppose you are engineering a mouse with a homozygous knockout of gene M as dicussed in chapter 10(gene replacement). You have completed many steps in the process and now, you are screening mouse pups for those that are heterozygous for the knockout – i.e., mice that have the wild type M allele on one chromosome, and the disrupted m allele on the other chromosome. To test a given mouse, you extract its genomic DNA, cut it with EcoRI, and perform a southern blot. A diagram of the wild type and knockout chromosomes is shown below. The position where the probe hybridizes is also shown. Your southern analysis includes mice of all possible genotypes – MM, Mm, and mm. Draw the banding pattern you would expect for each genotype on blot A below. Suppose the probe hybridizes only to the inserted DNA (the grey box on m chromosome). Draw the banding pattern you would expect for each genotype on blot B above.
