Question: After the murA gene is cloned into the vector, chemically
competent E. coli cells are transformed…



After the murA gene is cloned into the vector, chemically
competent E. coli cells are transformed with the pET11a-murA vector
using the heat shock method. This is a process where the cells are
stressed out by being transferred from 4 °C to 42 °C, causing them
to take up exogenous DNA, including the pET11a-murA vector. After
the heat shock, cells are allowed to recover at 37 °C for 1 hour
before being plated. Which media type will the cells need to be
plated on? 4. Which media type will the cells need to be plated
on?

a. LB complete media

b. LB complete media + ampicillin

c. LB complete media + oriC

d. LB complete media + lactose

e. LB complete media + BamHI

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