Question: In E. coli the σ70 subunit is involved in
transcription initiation. There are about 700 molecules…
In E. coli the σ70 subunit is involved in
transcription initiation. There are about 700 molecules of the
σ70 subunit and 1 molecule of the chromosome per cell.
The σ70 subunit binds to the RNA polymerase/promoter
complex with a Kd of 0.29 nM. If an E. coli cell has dimensions of
1 μm x 2μm, calculate the concentrations of the σ70
subunit and the chromosome to support the idea that the affinity
the σ70 subunit has for the RNA polymerase/promoter
complex is reasonable. Explain your results with regard to
equilibrium binding between these components.
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