Question: Correctly identify each of the studies below as either a forward or reverse genetics approach. T…
Show transcribed image text Correctly identify each of the studies below as either a forward or reverse genetics approach. To identify genes involved in ovarian cancer, you treat mice with EMS and look for A. Forward genetics mice with tumorous ovaries. Then you identify mutations that cause the tumors and analyze the molecular functions of the genes they affect. B. Reverse Genetics To analyze the function of BRCA1, a gene that influences susceptibility to breast and ovarian cancers, you produce strains of mice that have mutant alleles of BRCA1 and analyze their phenotypes To identify genes involved in heart disease, you look for QTL that are associated with heart disease in humans. You analyze the molecular functions of genes within each QTL. To uncover the genetic basis of feeding in Drosophila, you mutagenize the flies with P-element DNA transposons, and identify flies that prefer to eat pizza over the standard Drosophila food. You identify the genes that are mutated in the pizza eating flies and analyze their molecular function. our research is generously funded by Papa John's. The gene sonic hedgehog (SHH) was first identified in Drosophila, because loss of function mutations in SHH disrupted larval development, producing larvae that looked like hedgehogs. To analyze the function of SHH in mice, you produce loss of function mutations and examine the development of the mutant animals. To uncover the genetic pathway involved in glucose metabolism in a newly discovered species of bacteria, you treat bacteria with EMS and screen for colonies that grow on standard media, but not on media lacking glucose. You identify the causative mutations and analyze the molecular functions of a genes that are affected. CFTR codes for the Cystic Fibrosis Transmembrane Receptor. Humans with mutations is CFTR develop cystic fibrosis, a genetic disease that affects the lungs, pancreas, kidney, liver and intestines. To identify the particular cell types and developmental stages where CFTR is expressed, you produce a transcriptional fusion protein where green flourescent protein (GFP), is regulated by the promoter and cis- regulatory elements that determine CFTR expression.
Correctly identify each of the studies below as either a forward or reverse genetics approach. To identify genes involved in ovarian cancer, you treat mice with EMS and look for A. Forward genetics mice with tumorous ovaries. Then you identify mutations that cause the tumors and analyze the molecular functions of the genes they affect. B. Reverse Genetics To analyze the function of BRCA1, a gene that influences susceptibility to breast and ovarian cancers, you produce strains of mice that have mutant alleles of BRCA1 and analyze their phenotypes To identify genes involved in heart disease, you look for QTL that are associated with heart disease in humans. You analyze the molecular functions of genes within each QTL. To uncover the genetic basis of feeding in Drosophila, you mutagenize the flies with P-element DNA transposons, and identify flies that prefer to eat pizza over the standard Drosophila food. You identify the genes that are mutated in the pizza eating flies and analyze their molecular function. our research is generously funded by Papa John's. The gene sonic hedgehog (SHH) was first identified in Drosophila, because loss of function mutations in SHH disrupted larval development, producing larvae that looked like hedgehogs. To analyze the function of SHH in mice, you produce loss of function mutations and examine the development of the mutant animals. To uncover the genetic pathway involved in glucose metabolism in a newly discovered species of bacteria, you treat bacteria with EMS and screen for colonies that grow on standard media, but not on media lacking glucose. You identify the causative mutations and analyze the molecular functions of a genes that are affected. CFTR codes for the Cystic Fibrosis Transmembrane Receptor. Humans with mutations is CFTR develop cystic fibrosis, a genetic disease that affects the lungs, pancreas, kidney, liver and intestines. To identify the particular cell types and developmental stages where CFTR is expressed, you produce a transcriptional fusion protein where green flourescent protein (GFP), is regulated by the promoter and cis- regulatory elements that determine CFTR expression.
