Question: Viruses can be used by scientists as vectors to introduce genes into a human host cell. Which of …
Show transcribed image text Viruses can be used by scientists as vectors to introduce genes into a human host cell. Which of the following statements is/are correct about this topic? The retroviruses that are used have a membrane that allow them to fuse with the membranes of the host cell. Engineered retroviruses are used, because they have reverse transcriptase, and thus the ability to make a DNA copy of their RNA genome (including an RNA copy of the human gene that scientists are working with) and the enzymes to splice the resulting DNA into the host cell chromosome. DNA viruses are used, so that a human gene can be spliced directly into the virus DNA, and then injected into the host cell along with the viral DNA. When used as vectors, viruses carry smaller pieces of DNA than a plasmid can carry. Save Fig. 16.8, and the tutorial text around it, describing "sticky ends" of DNA, shows that DNA from both sources must have the same palindromic recognition sequence. shows that DNA molecules from 2 different sources, if cut with the same restriction enzyme, always form a recombinant DNA molecule. shows a homologous pair of chromosomes. shows that DNA from two sources (e.g., a bacterium and a jellyfish), if cut with the same restriction enzyme, have sticky ends that can match up through base-pairing. Save Scientists have attempted gene therapy (the insertion of good copies of genes missing or mutated in genetic disease patients) for a variety of genetic diseases, including SCID (severe combined immunodeficiency). Which of the following statements is correct about this topic? Save The disease affects primarily boys because boys have only one copy of the T cell receptor gene whose mutation causes the disease. The scientists did the risky gene therapy experiment on 10 boys for whom no bone marrow match could be found, because there was little hope that these boys would otherwise survive. Even close relatives may not be a close enough match to donate bone marrow to a sufferer of SCID; even slight genetic differences between siblings, for example, can be enough to trigger tissue rejection in the recipient. Bone marrow transplants from close relatives can be a cure, because with the transplanted bone marrow, the babies receive cells with the normal genes for the T cell receptor. the introduction to the Tutorial, an example of a use for recombinant DNA technology to solve the problem of safe landmine removal. In this example, a recombinant DNA molecule was made that included DNA sequences from 3 different species, bacteria contributed a gene promoter that was activated by the presence of TNT (dynamite) in the soil, jellyfish were crossed (mated) with bacteria, a bacterial promoter turned on a plant gene.
Viruses can be used by scientists as vectors to introduce genes into a human host cell. Which of the following statements is/are correct about this topic? The retroviruses that are used have a membrane that allow them to fuse with the membranes of the host cell. Engineered retroviruses are used, because they have reverse transcriptase, and thus the ability to make a DNA copy of their RNA genome (including an RNA copy of the human gene that scientists are working with) and the enzymes to splice the resulting DNA into the host cell chromosome. DNA viruses are used, so that a human gene can be spliced directly into the virus DNA, and then injected into the host cell along with the viral DNA. When used as vectors, viruses carry smaller pieces of DNA than a plasmid can carry. Save Fig. 16.8, and the tutorial text around it, describing "sticky ends" of DNA, shows that DNA from both sources must have the same palindromic recognition sequence. shows that DNA molecules from 2 different sources, if cut with the same restriction enzyme, always form a recombinant DNA molecule. shows a homologous pair of chromosomes. shows that DNA from two sources (e.g., a bacterium and a jellyfish), if cut with the same restriction enzyme, have sticky ends that can match up through base-pairing. Save Scientists have attempted gene therapy (the insertion of good copies of genes missing or mutated in genetic disease patients) for a variety of genetic diseases, including SCID (severe combined immunodeficiency). Which of the following statements is correct about this topic? Save The disease affects primarily boys because boys have only one copy of the T cell receptor gene whose mutation causes the disease. The scientists did the risky gene therapy experiment on 10 boys for whom no bone marrow match could be found, because there was little hope that these boys would otherwise survive. Even close relatives may not be a close enough match to donate bone marrow to a sufferer of SCID; even slight genetic differences between siblings, for example, can be enough to trigger tissue rejection in the recipient. Bone marrow transplants from close relatives can be a cure, because with the transplanted bone marrow, the babies receive cells with the normal genes for the T cell receptor. the introduction to the Tutorial, an example of a use for recombinant DNA technology to solve the problem of safe landmine removal. In this example, a recombinant DNA molecule was made that included DNA sequences from 3 different species, bacteria contributed a gene promoter that was activated by the presence of TNT (dynamite) in the soil, jellyfish were crossed (mated) with bacteria, a bacterial promoter turned on a plant gene.
