Question: 1.Draw a representative chromatogram depicting the probable
order of elution of lysine Glycine an…



1.Draw a representative chromatogram depicting the probable
order of elution of lysine Glycine and glutamic acid from a DowEX
50 cation exchange resin subject to stepwise elution from pH 3.0,
to PH 6.0, to PH 9.0.

Note: consider the prevailing ionic structures of each amino
acid at each ph indicated while formulating your response. You may
assume that the Dowex 50 resin bears a net negative charge under
all conditions specified

subject:biochemical analysis

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