Question: For a laboratory project you asked to purify the enzyme lactate dehydrogenase (LDH). The first st…

For a laboratory project you asked to purify the enzyme lactate
dehydrogenase (LDH). The first step of this project is to prepare
the lysis buffer that you will use to extract the enzyme. The
protocol you are following says the LDH lysis buffer should
contain:

25 mM CAPS (a pH buffer) at pH 10
5 mM

MgCl2 0.2%

Triton X-100 (a
detergent)

10 µg/ml pepstatin A (a protease
inhibitor)

You find the following reagents
on the shelves in lab:

a bottle of CAPS powder; MW is
221.2, pKa is 10.4

a bottle of MgCl2 crystals; MW is
95.2

a bottle of Triton X-100 (this
detergent is a liquid in its pure state)

and a stock solution of 25 mg/ml
pepstatin A

You need to make 200 ml of this
buffer. Tell how you would make this solution if you were to
actually make it in the lab.