Question: You are studying a protein that contains the peptide sequence
RDWKLVI. The part of the DNA encodi…
You are studying a protein that contains the peptide sequence
RDWKLVI. The part of the DNA encoding this peptide is included in
the sequence shown below. 5?-GGCGTGACTGGAAGCTAGTCATC-3?
3?-CCGCACTGACCTTCGATCAGTAG-5? This sequence does not contain any
HindIII restriction enzyme sites, but you want such a site for ease
of cloning. The target sequence for the HindIII restriction
nuclease is shown below. Your goal is to create a HindIII site on
this DNA without changing the coding sequence of the protein.
Indicate on the DNA sequence where you would do this and explain (a
few sentences).