Question: Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compo…

Ion Exchange Chromatography Experiment

1. What is the basis for the seperation of different
compounds by ion exchange?

2. How can molecules with the same charge at varying
amounts be seperated by chromatography?

3. Why are celluloses often used as supports to
seperate large biological ly active proteins?

4. Why is it important to prepare a standard curve for
each spectrophotometer?

5. What would happen if 0.5M K+ acetate were used to
elute the sample? Why?
(0.01M KOAc was actually used)