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Question

Ligand-Receptor interactions

a.What method(s) do you use, and how do
you analyze the data, when you want to know the AFFINITY of a
receptor

a.for a radioligand

b.for a ligand that is ONLY available
unlabeled, or has too low an affinity to be trapped in a binding
assay if it were radiolabeled

b.What method(s) do you use, and how do
you analyze the data, when you want to know the density of a
receptor population?

c.What method(s) do you use, and how do
you analyze the data, when you want to know the complexity of
binding of a radioligand? And what data outcomes would you
expect?

d.What method(s) do you use, and how do
you analyze the data, when you want to know the complexity of
binding of an UN-labeled ligand at a receptor? And what data
outcomes would you expect?

e.What attributes of binding can be
uniquely revealed by examining radioligand dissociation via
‘infinite dilution’ versus dissociation in the presence of 100-1000
–fold excess unlabeled ligand?