Question: You
have designed a forward and reverse primer to detect a circular
piece of DNA. In addition to …
You
have designed a forward and reverse primer to detect a circular
piece of DNA. In addition to the primers what other components are
required for the PCR reaction to amplify the DNA.
have designed a forward and reverse primer to detect a circular
piece of DNA. In addition to the primers what other components are
required for the PCR reaction to amplify the DNA.
a. taq DNA polymerase only, in appropriate buffer
b. taq DNA polymerase and NTPs in appropriate buffer
c. taq DNA polymerase and dNTPs in appropriate buffer
d. E.Coli DNA Pol I oth dNTPs and Mg2+ in appropriate
buffer
buffer
e. taq DNA polymerase, dNTP and Mg2+ in appropriate
buffer
buffer
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