Question: II. Consider a preparation that
contains all the enzymes and cofactors necessary for the
biosynth…



Question: II. Consider a preparation thatcontains all the enzymes and cofactors necessary for thebiosynth...II. Consider a preparation that
contains all the enzymes and cofactors necessary for the
biosynthesis of palmitate from added acetyl-CoA and malonyl-CoA.
(a) If [2-14C-2-3H]acetyl-CoA (see the left figure above) and an
excess of unlabeled malonyl-CoA are added as substrates, how many
3H atoms are incorporated into every molecule of palmitate, and
what are their locations? How many 14C atoms are incorporated and
what are their locations? (b) If unlabeled acetyl-CoA and an excess
of [1,3-14C-2-3H]malonyl-CoA (see the right figure above) are added
as substrates, how many 3H atoms are incorporated into every
molecule of palmitate, and what are their locations? How many 14C
atoms are incorporated and what are their locations? (c) Suppose
that both of the labeled molecules above were added as substrates,
how many 3H atoms are incorporated into every molecule

Show transcribed image text 3H 3H O O2 3H-14C-C 3 SCOA 3 H SCOA

3H 3H O O2 3H-14C-C 3 SCOA 3 H SCOA

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