Question: You have cloned a human gene along with its promoter and ribosome-binding site in a bacterial clo…

You have cloned a human gene along with its promoter and
ribosome-binding site in a bacterial cloning vector and then
introduced the recombinant plasmid into a bacterial host cell that
supports the replication and segregation of the cloning vector.
Unfortunately, even though you know the whole human gene sequence
is present (by DNA hybridization); you cannot get expression of the
gene. Which of the following are reasons why there is no
expression?

A. The vector cannot
replicate

B. The human promoter is not recognized by the
bacterial host cell

C. The ribosome-binding site is
not recognized by the bacterial host cell

D. The human gene lacks introns,
so it is not transcribed by the host bacterium

E. All the above

F. Answers A & C

G. Answers B & C