Question: Your cousin has been working on a high school science fair project in which she’s separating prot…

Your cousin has been working on a high school science fair
project in which she’s separating proteins from platypus milk.
She’s looking to you for guidance. In a few short paragraphs,
explain to her how she might use chromatography to achieve protein
separation. Assume the proteins in platypus milk range from 5 kD to
200 kD, and she’s interested in a protein that has a molecular
weight of 40 kD and is positively charged at neutral pH. There are
other 40 kD proteins in the milk, but they are negatively charged
at neutral pH. (There are also many proteins in the milk that are
also positively charged, but of different size.) In your answer,
describe and recommend two different types of chromatography.
Explain the theoretical basis of separation for each technique and
describe the resin she should use to isolate the protein and the
expected results for the separation.