Question: Suppose you have a DNA fragment you would like to insert into the pSAP plasmid. The fragment has …
Show transcribed image text Suppose you have a DNA fragment you would like to insert into the pSAP plasmid. The fragment has Pstl and EcoRI restriction endonuclease sites near the 5' end and Hindlll and restriction endonuclease sites near the 3' end. Choose the best restriction endonuclease(s) to digest the both the DNA fragment and pSAP. EcoRI and Smal EcoRI only EcoRI and Hindlll Psfl and Hindlll PsA and Small Psfl only
Suppose you have a DNA fragment you would like to insert into the pSAP plasmid. The fragment has Pstl and EcoRI restriction endonuclease sites near the 5' end and Hindlll and restriction endonuclease sites near the 3' end. Choose the best restriction endonuclease(s) to digest the both the DNA fragment and pSAP. EcoRI and Smal EcoRI only EcoRI and Hindlll Psfl and Hindlll PsA and Small Psfl only
