Question: 2-Outline the steps from starting tissue to final image for an antibody stain a. mRNA extraction …

2-Outline the steps from starting tissue to final image for an
antibody stain

a. mRNA extraction

b. cDNA synthesis

c. Primer design and gene amplification

d. Gene cloning

e. Target Protein synthesis

f. Antibody production

g. Antibody staining (including the role of 1° and 2°
antibodies)