Question: Answer the following four questions about supercoiling and
linking number:
a) What is the linking…



Answer the following four questions about supercoiling and
linking number:

a) What is the linking number, Lk, for a relaxed,
closed-circular DNA with 3675 base pairs? Enter your answer as an
integer.

b) What is Lk for negatively supercoiled 3675 bp DNA if it is
underwound by 2 complete turns?

c) How does Lk change when there is a break in one strand?

a. It decreases by the number of strands broken

b. It is undefined

c. It increases by 1

d. It decreases by the number of turns removed from the
molecule

d) What is Lk for negatively supercoiled 3675 bp DNA after
treatment with one molecule of topoisomerase II (DNA gyrase) and
adequate ATP?

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