Question: Consider a preparation that contains all the enzymes and
cofactors necessary for the biosynthesis…
Consider a preparation that contains all the enzymes and
cofactors necessary for the biosynthesis of palmitate from added
acetyl-CoA and malonyl-CoA.
(a) If [2-14C-2-3H]acetyl-CoA (see the left figure above) and an
excess of unlabeled malonyl-CoA are added as substrates, how many
3H atoms are incorporated into every molecule of palmitate, and
what are their locations? How many 14C atoms are incorporated and
what are their locations?
(b) If unlabeled acetyl-CoA and an excess of
[1,3-14C-2-3H]malonyl-CoA (see the right figure above) are added as
substrates, how many 3H atoms are incorporated into every molecule
of palmitate, and what are their locations? How many 14C atoms are
incorporated and what are their locations?
(c) Suppose that both of the labeled molecules above were added
as substrates, how many 3H atoms are incorporated into every
molecule
