Question: Consider a preparation that contains all the enzymes and
cofactors necessary for the biosynthesis…



Consider a preparation that contains all the enzymes and
cofactors necessary for the biosynthesis of palmitate from added
acetyl-CoA and malonyl-CoA. (a) If [2-14C-2-3H]acetyl-CoA (see the
left figure above) and an excess of unlabeled malonyl-CoA are added
as substrates, how many 3H atoms are incorporated into every
molecule of palmitate, and what are their locations? How many 14C
atoms are incorporated and what are their locations? (b) If
unlabeled acetyl-CoA and an excess of [1,3-14C-2-3H]malonyl-CoA
(see the right figure above) are added as substrates, how many 3H
atoms are incorporated into every molecule of palmitate, and what
are their locations? How many 14C atoms are incorporated and what
are their locations? (c) Suppose that both of the labeled molecules
above were added as substrates, how many 3H atoms are incorporated
into every molecule of palmitate, and what are their locations? How
many 14C atoms are incorporated and what are their locations?

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