Question: I really just don’t know how to answer these kinds of questions,
and they appear a lot. I would a…
I really just don’t know how to answer these kinds of questions,
and they appear a lot. I would appreciate help understanding
restriction maps.
Show transcribed image text 也OutsideCove × もbiol207 prac X 也biol207 prac × 也Course: BIOL 也Biology 207. × 也b207answer: X。Restrictio e Home | Chec 3 Ways to Ta x × × × ← https://eclass.sr.ualberta.ca/pluginfile.php/2536951/mod_resource/content/1/biol207-practice-final.pdf Question 10 Stratagene makes a kit that turns mRNAs into cDNAs in such a way that the cDNAs have an EcoRl sticky end at the start codon end and a Xhol sticky end at the stop codon end. The cDNAs can then be subcloned into pBluescript using the techniques you used in the lab. You have purchased a recombinant plasmid made this way that should contain the cDNA of your favourite gene (yfg). To confirm this you do restriction digests and agarose gel electrophoresis. Use these maps to draw the bands you expect to see in this gel. Label each band with its approximate size to make it easier to mark. (5 marks) Note that yfg is 9 kb in total our Tuu our gona EcoRI XhoI XhoI DNA MRVA pBluescrip total size 3 kb Xho EcoRI mp or 7:01 PM I'm Cortana. Ask me anything 恤脈 )) ENG 2016-04-14
也OutsideCove × もbiol207 prac X 也biol207 prac × 也Course: BIOL 也Biology 207. × 也b207answer: X。Restrictio e Home | Chec 3 Ways to Ta x × × × ← https://eclass.sr.ualberta.ca/pluginfile.php/2536951/mod_resource/content/1/biol207-practice-final.pdf Question 10 Stratagene makes a kit that turns mRNAs into cDNAs in such a way that the cDNAs have an EcoRl sticky end at the start codon end and a Xhol sticky end at the stop codon end. The cDNAs can then be subcloned into pBluescript using the techniques you used in the lab. You have purchased a recombinant plasmid made this way that should contain the cDNA of your favourite gene (yfg). To confirm this you do restriction digests and agarose gel electrophoresis. Use these maps to draw the bands you expect to see in this gel. Label each band with its approximate size to make it easier to mark. (5 marks) Note that yfg is 9 kb in total our Tuu our gona EcoRI XhoI XhoI DNA MRVA pBluescrip total size 3 kb Xho EcoRI mp or 7:01 PM I'm Cortana. Ask me anything 恤脈 )) ENG 2016-04-14
