Question: . In a study by Van Dyck et al. (1999), purified human Rad52 protein, which is important for DSB …

. In a study by Van Dyck et al. (1999), purified human
Rad52 protein, which is important for DSB repair and homologous
recombination in many eukaryotes, was added to linear DNA
internally labeled with ³²P (Figure 3 attached pdf). The
DNA bound with saturating amounts of hRad52 or not was treated with
an exonuclease or an endonuclease. DNA degradation is monitored by
the production of ³²P-labeled DNA fragments that are
soluble in 10% trichloroacetic acid.  

– Briefly, explain your rationale for where Rad52 binds to
linear DNA.

– Name the earliest step during DSB repair/recombination where
Rad52 could bind in vivo?