Question: 2. A Calcite Sample Yields The Following Data For The Determination Of Calcium As CaO: 55.95, 56.00, 56.04. 56.08, And 56.23. Should We Reject 56.23? (2.5 Marks) 3. A Standard Curve For Protein Concentration Is Often Created Using Known Concentrations Of Bovine Serum Albumin. The Assay Is Called The Bradford Assay; It Is A Colorimetric Assay. The Reagent …

2. A calcite sample yields the following data for the determination of calcium as CaO: 55.95, 56.00, 56.04. 56.08, and 56.23. Should we reject 56.23? (2.5 Marks) 3. A standard curve for protein concentration is often created using known concentrations of bovine serum albumin. The assay is called the Bradford assay; it is a colorimetric assay. The reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (A device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and the following measurements were obtained using a spectrophotometer: (5 Marks) 0.58 Protein Concentration (mg/ml) Absorbance (A) 0.29 0.07% 0.143 0.83 0.193 0.473 1.42 0.527 c. Guideline to proceed: Create a graph of the data that is appropriately titled and labeled (Include your name on the graph) b. Use a straight edge to sketch a line that “best” approximates the trend of this data. Estimate the slope and y-intercept and use them to find the equation of the associated line using the slope-intercept form of a line: y=mx+c d. Pick two points on the line that you’ve drawn [(x1.yl) and (x2,y2)) and use these points to find the equation of this line. Determine the protein concentrations of the following unknown samples: Absorbance (A) Protein Concentration (mpmilj e. 0.027 0.205 0.632 0.750 Show your work.