You are performing an experiment in which you will be removing a2mm piece of tissue from the spinal cord of mice. Your goal is tostudy the ability of stem cell delivery to fill in this gap andpromote recovery and repair after this type of an injury. You alsoplan to eventually use the data from this study to perform clinicaltrials in individuals suffering from stab wounds at the spinallevel.
- What cell type would you transplant? (Be specific; i.e., fromwhich species, pluri or multi or uni, what lineage, etc.).
- Why did you choose this cell population?
- How would you test your cells in vitro to make sure theyfunction properly before transplanting them? Name 4 tests you wouldperform. (No explanations needed)
- What is the most important culture condition in which the cellsneed to be maintained prior to transplantation and why?
- Based on what you have learned in this class, how many daysafter the removal of the 2mm spinal cord tissue should the cells betransplanted to maximize recovery and why?
- Assuming that you find the animals receiving your cells walkbetter than your control group, what are 2 possible mechanisms forthe recovery?
- What will you need to receive FDA authorization for a Phase Iclinical trial?
- Assuming that you receive approval for your clinical trials, inPhase I, approximately how many patients will you recruit?
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