Question: Question 10 Putting It All Together With Common Molecular Biology Buffers A Very Commonly Used Buffer Is PBS – Phosphate Buffered Saline. This Buffer Is Isotonic Meaning It Exerts The Same Osmotic Pressure As The Solution In The Inside Of A Cell. If You Resuspend Cells In PBS They Won’t Burst, Which Would Happen If You Put Them Into Distilled Water. …

Question 10 Putting it all together with common molecular biology buffers A very commonly used buffer is PBS – phosphate buffered saline. This buffer is isotonic meaning it exerts the same osmotic pressure as the solution in the inside of a cell. If you resuspend cells in PBS they won’t burst, which would happen if you put them into distilled water. You have a recipe for 10 X PBS: Dissolve 80 g NaCl (mol. Wt. 58.4), 26.8 g Na2HPO4-7H20 (mol. Wt. 268) and 2.4 g KH2PO4 (mol. Wt. 136) in 800 mL water. Adjust pH to 7.4 with HCl then adjust final volume to 1 L with water. Sterilise by autoclaving. What is the composition of PBS? I