Gene Expression Worksheet
This sequence below is double-stranded DNA, and is part of agene. Assume the promoter is on the left, and transcriptionproceeds left to right, using the bottom strand of the DNA as thetemplate. Once transcribed into RNA, use the genetic code table (onthe back, or from your textbook) to create an amino acid (Protein)sequence.
1) 5’ CGATGGAACTATTGGGTAATGTATAA 3’
3’ GCTACCTTGATAACCCATTACATATT 5’
RNA:
Protein:
Now, what would happen if a mutation changed the DNA in thefollowing way?:
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2) 5’ CGATGGCACTATTGGGTAATGTATAA 3’
3’ GCTACCGTGATAACCCATTACATATT 5’
RNA:
Protein:
What kind of mutation is this (compare to first sequence)?
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3)What would happen if a mutation changed the DNA in thisway?:
5’ CGATGGAACTATAGGGTAATGTATAA 3’
3’ GCTACCTTGATATCCCATTACATATT 5’
RNA:
Protein:
What kind of mutation is this (compare to first sequence)?
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4)What would happen if a mutation changed the DNA in thefollowing way?:
5’ CGATGGAGACTATTGGGTAATGTATAA 3’
3’ GCTACCTCTGATAACCCATTACATATT 5’
RNA:
Protein:
What kind of mutation is this (compare to first sequence)?