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Biochemical tests, part Positive and negative controls are given as a reference for each test. Observation means what you see and interpretation is the meaning of that observation. Interpretation does not mean a’ or result Positive for mannitol fermentation POS-Staphylococcus aureus NEG-S. epidermidis Media changed to yelow Media stayed pink 1. MANNITOL Negative for mannitol fermentation FERMENTATION SKIN SAMPLE Positive for B-galactosidase enzyme Liquid changed to yellow POS- Staphylococcus 2. ONPG intermedius (3-Galactosidase NEG. Staphylococcus aureus Activity) Liquid stayed dear Negative for B-galactosidase enzyme SKIN SAMPLE Motility POS- E. col Cloudy throughout and near the stab line 3. MOTILITY No motility NEG-Klebsiella pneumoniae The stab ine is clear (SIM TUBE) SKIN SAMPLE POS Proteus mirabilis Blackening of media Production of hydrogen sulfide 4. H2S Sulfur reduction NEG. Pseudomonas aeruginosa No darkening of media No production of hydrogen sulfide SKIN SAMPLE (SIM TUBE) POS- Klebsiella pneumoniae Color change to bright blue Carbon as an energy source NEG Yersinia Media stayed green No use of carbon as an energy source 5. CITRATE SKIN SAMPLE 6. GELATINASE POS. Serratia Media turned to liquid Positive for gelatinase enzyme cas, parti NEG-Citrobacter Media stayed solid Negative for gelatinase enzyme SKIN SAMPLE Positive for mannitol fermentation Media changed to yellow 7. MANNOSE Media stayed pink Negative for mannitol fermentation FERMENTATION SKIN SAMPLE 8. Based on the results above and all your selective and differential media results, summarize all the biochemical characteristics of your SKIN isolate: 2 ONPG SIM Gelatin NNOSB Citrate pheno OL 0.
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