Question: 1) Homologous recombination can be used in the lab to replace a gene of interest with a selection…

1) Homologous recombination can be used in the lab to replace a
gene of interest with a selection marker.

What is required for the selection marker to specifically
replace the gene of interes

A. The sequences surrounding the selection marker will be placed
around the gene of interest

B. It depends on the media used to test for incorporation of the
selection marker

C. The sequences surrounding the gene of interest will be placed
around the selection marker.

D. Specific selection markers are only capable of replacing
specific genes.

2) Homologous recombination can be used in the lab to replace a
gene of interest with a selection marker. To confirm that the
selection marker is integrated into the correct place in the
genome, sometimes a negative selection is used in addition to the
standard positive selection marker. Which of the following would
act as a negative selection marker?

A) Transformation of a different gene, which does not act as a
selection marker, to replace the gene of interest.​

B) A second marker in the knock out cassette, that if inserted
into the genome results in cell death when plated on selective
media.

C) Transforming the cells with two different knock out
cassettes, each containing the same selection marker3. ​

3. Why is repairing a one stranded break more efficient than
repairing a double stranded break?

.​A It isn’t caused by a phosphodiester bond hydrolysis

B. doesnt require ligase

c. Doesnt result in a loss of nuceltoides