Question: 1)What features are needed in an expression vector that are not necessary in a simple cloning vec…

1)What features are needed in an expression vector that are not
necessary in a simple cloning vector?

2)How do you decide which type of vector to use (plasmid, BAC,
YAC)?

3)How is protein expression (in E. coli) induced using the lac
system?

4)There are several chromotographic options for purifying
protein from lysed cells. what are the characteristics of the
column and of the molecules being purified, of chromatographc
options?

5)How does a native gel compare to an SDS gel? What information
can be derived from an SDS gel?