Question: 3-Outline the steps from starting tissue to final image for an
in situ hybridization
a. mRNA extr…
3-Outline the steps from starting tissue to final image for an
in situ hybridization
a. mRNA extraction
b. cDNA synthesis
c. Primer design and gene amplification
d. Gene cloning
e. Riboprobe synthesis
f. In situ hybridization (including fluorescent and colormetric
methods)
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