A 28 year old man was screened as a potential blooddonor. He is a healthy donor with unremarkable medical history andno medication. A Medical Technologist performed direct typing as aroutine test for the screening. The reverse typing request wasadded later; during this time the Medical Technologist was swampedwith test requests to perform so she did the procedure as fast asshe could. The initial ABO blood typing results are asfollow:

ABO Testing Results

Donor Red Cells with

Donors Serum with Reagent Cells

Anti-A

Anti-B

A1

B

0

0

0

0

QUESTIONS:

1. Evaluate the ABO results. Are the reactions in theblood typing typical/unusual/ expected to occur?Explain

2. As a quality control, what should the MedicalTechnologist do to establish the correct blood type of thepatient?

3. What could be the possible cause/s of the ABOdiscrepancy in this particular case?

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