Question: A circular bacterial plasmid (pBPl) has a single HindIII restriction-enzyme site in the middle of…
Show transcribed image text A circular bacterial plasmid (pBPl) has a single HindIII restriction-enzyme site in the middle of a tetracycline-resistance gene (ter^R). Fruit fly genomic DNA is digested with/find, and a library is made in pBPl. Probing reveals that clone 15 contains a specific Drosophila gene of interest. Clone 15 is studied by restriction analysis with Hindlll and another restriction enzyme, coRV. The cthidium bromide-stained electrophoretic gel shows bands as in the accompanying diagram (the control was an uninserted plasmid pBPl). The sizes of the bands (in kilobascs) are shown alongside, a. Draw restriction maps for plasmid pBPl with and without the insert, showing the sites of the target sequences and the approximate position of tet^R gene. b. If the same tet^R gene cloned in a completely nonhomologous vector is made radioactive and used as a probe in a Southern blot of this gel, which bands do you expect to appear radioactive on an autoradiogram?
A circular bacterial plasmid (pBPl) has a single HindIII restriction-enzyme site in the middle of a tetracycline-resistance gene (ter^R). Fruit fly genomic DNA is digested with/find, and a library is made in pBPl. Probing reveals that clone 15 contains a specific Drosophila gene of interest. Clone 15 is studied by restriction analysis with Hindlll and another restriction enzyme, coRV. The cthidium bromide-stained electrophoretic gel shows bands as in the accompanying diagram (the control was an uninserted plasmid pBPl). The sizes of the bands (in kilobascs) are shown alongside, a. Draw restriction maps for plasmid pBPl with and without the insert, showing the sites of the target sequences and the approximate position of tet^R gene. b. If the same tet^R gene cloned in a completely nonhomologous vector is made radioactive and used as a probe in a Southern blot of this gel, which bands do you expect to appear radioactive on an autoradiogram?
