Question: A.) How is cDNA is prepared B.) Describe a plasmid and relate it to the concept of a cloning …

A.) How is cDNA is prepared

B.) Describe a plasmid and relate it to the concept of a cloning
vector.

C.) Describe how restriction enzymes work.

D.) Explain the steps in cloning the GH1 gene (

E.) Explain the steps involved in cell transformation

F.) Describe the process of screening a cDNA library

G.) Define the term ‘probe’.

H.)Describe the process of PCR.

I.) List the components of PCR

J.) Contrast ddNTPs with dNTPs

K.) Describe the basics of Sanger sequencing