Question: A northern Blot experiment is carried out with mRNA isolated from E. coli using a DNA probe corre…
Show transcribed image text A northern Blot experiment is carried out with mRNA isolated from E. coli using a DNA probe corresponding to the lacA gene. Wildtype lac operon mRNA is approximately 5 kb in length. You are studying the following mutants: nucleotide substitution that encodes inactive protein nucleotide substitution that encodes inactive protein deletion missing 2 kb of lacZ coding sequence, no protein produced deletion missing 0.5 kb of lacy coding sequence, no protein produced The mRNA from the following six genotypes is analyzed via Northern Blot after growth of the bacteria in either the presence (+) or absence (-) of inducer. For each genotype indicate on the gel where an mRNA band would be expected.
A northern Blot experiment is carried out with mRNA isolated from E. coli using a DNA probe corresponding to the lacA gene. Wildtype lac operon mRNA is approximately 5 kb in length. You are studying the following mutants: nucleotide substitution that encodes inactive protein nucleotide substitution that encodes inactive protein deletion missing 2 kb of lacZ coding sequence, no protein produced deletion missing 0.5 kb of lacy coding sequence, no protein produced The mRNA from the following six genotypes is analyzed via Northern Blot after growth of the bacteria in either the presence (+) or absence (-) of inducer. For each genotype indicate on the gel where an mRNA band would be expected.
