Question: A. owHow might the affinities of Protein –protein interactions in structural protein complexes di…

a. owHow might the affinities of
Protein –protein interactions in structural protein
complexes

differ from the affinities of Protein –protein
interactions among proteins in signal transduction
processes

b.What are the varieties of ways that
affinities for differing protein-protein interactions could be
modulated?

c.What additional information can be
learned in a mammalian cell expression readout for a library –based
bait identification that cannot be learned by a yeast two-hybrid
library based bait identification?

d.When a protein is co-precipitated in
an antibody-based Co-IP assay, is this definitive confirmation that
the protein targeted by the antibody in the IP assay is
directly interacting with the protein co-isolated in the
complex? Why or why NOT ?

e.Compare and Contrast the Bir-A
non-specific IgG isolation procedure for protein-protein complexes
with a Protein complementation assay, like that for
beta-galactosidase or other PCA assays.