Question: A single plate of adult stem cells (growing in two-dimensional culture) contains 8 million cells….
Show transcribed image text A single plate of adult stem cells (growing in two-dimensional culture) contains 8 million cells. A certain polymeric hydrogel scaffold will be used to "encapsulate" the stem cells in a certain tissue engineering approach. This is done by mixing 1 part (by volume) concentrated cell solution with 9 parts of an aqueous pre-polymer solution. When UV light is shined on the pre-polymer/cell suspension, it will cure into a gel-like material (much like a dental resin for filling cavities, but much softer and water-swollen). The molecular weight of the polymer is 3500 g/mol and it is a Tuesday and the relative humidity is 35%. You wish to make a total of twenty constructs. A construct has a total volume of 150 muL. That is, 150 muL. of pre-polymer/cell suspension is polymerized in each construct. The cells must be seeded in the construct at a concentration of 5 times 10^7 cells/mL How many plates of cells will you need to plan on having available for the cell encapsulation procedure? You cannot harvest fractions of a plate, so be sure to round your answer up to the nearest plate and show all work. At what concentration (cells per mL) must the cell suspension (i.e. the 1 part that is mixed with 9 parts pre-polymer solution) be brought to prior to the encapsulation procedure?
A single plate of adult stem cells (growing in two-dimensional culture) contains 8 million cells. A certain polymeric hydrogel scaffold will be used to "encapsulate" the stem cells in a certain tissue engineering approach. This is done by mixing 1 part (by volume) concentrated cell solution with 9 parts of an aqueous pre-polymer solution. When UV light is shined on the pre-polymer/cell suspension, it will cure into a gel-like material (much like a dental resin for filling cavities, but much softer and water-swollen). The molecular weight of the polymer is 3500 g/mol and it is a Tuesday and the relative humidity is 35%. You wish to make a total of twenty constructs. A construct has a total volume of 150 muL. That is, 150 muL. of pre-polymer/cell suspension is polymerized in each construct. The cells must be seeded in the construct at a concentration of 5 times 10^7 cells/mL How many plates of cells will you need to plan on having available for the cell encapsulation procedure? You cannot harvest fractions of a plate, so be sure to round your answer up to the nearest plate and show all work. At what concentration (cells per mL) must the cell suspension (i.e. the 1 part that is mixed with 9 parts pre-polymer solution) be brought to prior to the encapsulation procedure?
