Question: A wild-type culture of yast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells a…
Show transcribed image text A wild-type culture of yast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimum medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), autotrophic for one amino acid, or auxotrophic for more than one amino acid. His- Leu- prototrophic Arg- His-, Arg-, Leu-
A wild-type culture of yast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimum medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), autotrophic for one amino acid, or auxotrophic for more than one amino acid. His- Leu- prototrophic Arg- His-, Arg-, Leu-
