Question: A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells …
Show transcribed image text A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), auxotrophic for one amino acid, or auxotrophic for more than one amino acid.
A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), auxotrophic for one amino acid, or auxotrophic for more than one amino acid.
