Question: A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells …
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Show transcribed image text A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), auxotrophic for one amino acid, or auxotrophic for more than one amino acid. His- Leu- Arg- His-, Arg-, Leu-prototrophic
A wild-type culture of yeast is exposed to the mutagen ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid (plates 1-4) with the following results. Determine which colonies are prototrophic (wild-type), auxotrophic for one amino acid, or auxotrophic for more than one amino acid. His- Leu- Arg- His-, Arg-, Leu-prototrophic
