Question: Above is a list of 10 proteins
that represent the major contaminating proteins found in bacterial…
Above is a list of 10 proteins
that represent the major contaminating proteins found in bacterial
extracts. Also provided are the relevant functional characteristics
of these proteins and human glucokinase. Use this information to
develop a two-step purification procedure that will result in
purification of glucokinase. The first step of your purification
procedure should involve anion exchange chromatography. The second
step should involve size exclusion chromatography. In your answer,
be sure to indicate how each of these chromatography processes
work. Provide specific experimental details for your procedure
(i.e. what buffer and what pH). Indicate the order that each
protein in the mixture will elute.
Show transcribed image text Molecular weight kDa 124 35 28 50 82 18 110 69 8 91 42 Protein Isoelectri Point 8.8 6.7 8.4 7.2 6.8 5.9 7.2 4.6 8.8 6.3 7.5 T7 RNA polvmerase maltose binding protein carboxvpeptidase human glucokinasıe lactose permease actin lvcerol transporter lvcine tRNA svnthetase ribosomal protein L1 colicin binding protein adenosine deaminase
Molecular weight kDa 124 35 28 50 82 18 110 69 8 91 42 Protein Isoelectri Point 8.8 6.7 8.4 7.2 6.8 5.9 7.2 4.6 8.8 6.3 7.5 T7 RNA polvmerase maltose binding protein carboxvpeptidase human glucokinasıe lactose permease actin lvcerol transporter lvcine tRNA svnthetase ribosomal protein L1 colicin binding protein adenosine deaminase
