Question: After completing BIO325, you join a research lab and are asked to amplify a 5kb segment of DNA fr…

After completing BIO325, you join a research lab and are asked
to amplify a 5kb segment of DNA from double stranded genomic DNA
(the template DNA). You choose to use Taq Polymerase to amplify
your fragment and you are given primers with a melting temperature
(Tm) of 66?C. After performing PCR using the reaction conditions
below you did NOT see a product when you analyzed your PCR sample
by agarose gel electrophoresis.