Question: An F’ bacterial cell conjugated with an F- bacterial cell and
the following cells resulted: 1. Wh…
An F’ bacterial cell conjugated with an F- bacterial cell and
the following cells resulted: 1. What was the original
genotype of the donor cell (before conjugation)? 2. What was the
original genotype of the recipient cell (before conjugation)? 3.
Did recombination occur? Describe how you came to this conclusion.
4. If it did, draw out the recombination event. If it did not
occur, draw out the configurations of the chromosomes resulting in
non-recombinants. 5. You surveyed 1000 bacterial cells and found
recombination events between lac and thr occuring 28.7% of the
time, could you conclude that these genes are linked? Why or why
not? 6. Using the information above and the Hfr information below,
make a map of this bacterial chromosome making sure to label units
distances between genes whenever possible: Hfr1: Lac (5mins), Amp
(12 mins), Arg (32 min) Hfr2: Amp (3 mins), Lac (10 mins), Thr
(data lost)