Question: B. pUC18/19 is a commercially-available cloning vector that you could use to clone a DNA fragment…

b. pUC18/19 is a commercially-available cloning vector that you
could use to clone a DNA fragment that contains the entire coding
region.

Perform a search for a picture of the pUC18/19 cloning vector.
That picture should include a restriction map of the plasmid
vector, including the restriction sites in the multiple cloning
site.

i. Copy and paste the picture and print that page.

Then answer the following questions:

ii. Which enzyme(s) cut both within your sequence and also
within the multiple cloning site?

iii. Which enzyme(s) will allow you to clone the entire coding
region into the multiple cloning site of the vector?