Question: For a laboratory project you asked to purify the enzyme lactate dehydrogenase (LDH). The first st…

For a laboratory project you asked to purify the enzyme lactate
dehydrogenase (LDH). The first step of this project is to prepare
the lysis buffer that you will use to extract the enzyme. The
protocol you are following says the LDH lysis buffer should
contain: 25 mM CAPS (a pH buffer) at pH 10 5 mM MgCl2 0.2% Triton
X-100 (a detergent) 10 µg/ml pepstatin A (a protease inhibitor) You
find the following reagents on the shelves in lab: a bottle of CAPS
powder; MW is 221.3, pKa is 10.4 a bottle of MgCl2 crystals; MW is
95.2 a bottle of Triton X-100 (this detergent is a liquid in its
pure state) and a stock solution of 25 mg/ml pepstatin A You need
to make 200 ml of this buffer. Tell how you would make this
solution. Show your work.