Question: For in situ hybridization, the correct steps in order are: A. hybridize, permeabilize, add probe,…

For in situ hybridization, the correct steps in order are:

A. hybridize, permeabilize, add probe, fix, wash, primary
antibody, secondary antibody to DIG
B. fix, permeabilize, add probe, primary to DIG, hybridize, wash,
secondary antibody
C. fix, permeabilize, add probe, hybridize, wash, primary antibody
to DIG, secondary antibody
D. permeabilize, wash, add probe, hybridize, primary antibody to
DIG, fix
E. fix, permeabilize, treat with antibody, hybridize, wash, add
probe, primary antibody to DIG, secondary probe