Question: Hfr bacteria with the genotype lac+met+his+trp+thr+strS are mixed with F- bacteria with the genot…

Hfr bacteria with the genotype lac+met+his+trp+thr+strS are
mixed with F- bacteria with the genotype lac-met-his-trp-thr-strR.
Mating is interrupted at 20 minutes and replica plating is used to
determine the percentage of exconjugants that have the following
phenotypes.

Gene and percentage Of Colonies:

lac + 100%

trp+ 78%

thr+ 85%

his+ 67%

met+ 54%

a. what type of media should be used to select for exconjugants
(and ensure that none of the original Hfr and F- bacteria will
grow)?

b. draw a map of the bacterial chromosome from the Hfr strain
used in this interrupted mating experiment. Include the F plasmid
integration site as well as the direction and order of gene
transfer.