Question: High doses of caffeine interfere with the DNA replication checkpoint mechanism in mammalian cells…
Show transcribed image text High doses of caffeine interfere with the DNA replication checkpoint mechanism in mammalian cells. Why then do you suppose the U.S. Surgeon General has not yet issued a warning to heavy coffee and cola drinkers? {To answer this question, let's assume that a 150 ml cup of coffee contains 100 mg of caffeine (196 g/mol), and for the sake of the calculation, that you cannot measurably metabolize or excrete caffeine (which is not true) in the period of one day once you ingest it. How many cups of coffee would you have to drink in that day to reach the dose (10 mM) required to interfere with the DNA replication checkpoint mechanism? (A typical adult contains about 40 liters of water in total body fluid; we'll assume that you can excrete the water that you ingest, thus the total body fluid volume will remain constant at 40 liters.)}
High doses of caffeine interfere with the DNA replication checkpoint mechanism in mammalian cells. Why then do you suppose the U.S. Surgeon General has not yet issued a warning to heavy coffee and cola drinkers? {To answer this question, let's assume that a 150 ml cup of coffee contains 100 mg of caffeine (196 g/mol), and for the sake of the calculation, that you cannot measurably metabolize or excrete caffeine (which is not true) in the period of one day once you ingest it. How many cups of coffee would you have to drink in that day to reach the dose (10 mM) required to interfere with the DNA replication checkpoint mechanism? (A typical adult contains about 40 liters of water in total body fluid; we'll assume that you can excrete the water that you ingest, thus the total body fluid volume will remain constant at 40 liters.)}
