How do you do a cell split? 8.5 ml of Media of FBS was added to1.5 ml of trypsin in one plate. I was told to do a dilution factorof 1:15 with a 6 well plate. I was told to put 1 ml to each well onthe top of the plate, and 1.25 ml to the bottom of the plate. Whatis the logic of this? Also, what would a dilution factor of 3/20be? Thank you!
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