Question: How would you clone a gene into a plasmid if there were no
common restriction sites between the t…
How would you clone a gene into a plasmid if there were no
common restriction sites between the two
DNA sequences?
What enzyme is necessary to permanently link the digested
foreign and plasmid DNA together to form the
recombinant DNA molecule? How does this enzyme work?
What type of ends do the enzymes BamHI and EcoRI produce? How
does this type of end facilitate cloning?
What is the texture and consistency of the DNA?
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