Question: I need help identifying and drawing the graphs.
1)The following data describe the
affinities, in …
I need help identifying and drawing the graphs.
1)The following data describe the
affinities, in Kd values, of a radioligand and two unlabeled
ligands in interacting with two gene products, transmembrane
Receptor 1 and transmembrane Receptor 2 .
|
Receptor |
Radioligand 1 |
Unlabeled Ligand A |
Unlabeled Ligand B |
|
|
Receptor 1 |
Kd 0.2 nM |
Kd = 17 nM |
Kd= 60 nM |
|
|
Receptor 2 |
Kd 55 nM |
Kd = 17 nM |
Kd= 4 nM |
Show the Scatchard Plot OR, when requested, the
Competition Plot, with details about the position AND the
shape of the curve, under each of these
circumstanced
A: Radioligand Saturation Binding for Radioligand 1 when
added to a binding assay over the range of 0. 2 – 75
nM
B. Competition for 0.05 nM Radioligand binding 1 when
competed for by Ligand A and Ligand B ( in separate incubations)
added over the range of 0.2nM to 100 nM
C. Competition for 20 nM nM Radioligand binding 1 when
competed for by Ligand A and Ligand B ( in separate incubations)
added over the range of 0.2nM to 100 nM
A: Radioligand Saturation Binding for Radioligand 1 when
added to a binding assay over the range of 0.02 – 2.0
nM
